„How do I know if I have crushed a tick thoroughly?”

„As soon as the fluid changes color it is an indication that the contents of the stomach has emptied and the intestine was crushed.”

„Is a faint test line already considered as positive, if I only see it by holding the cassette slightly slanted?”

„Exactly, if only a few Borrelia have been in the tick the test line will be very faint, but the result has to be considered as positive.”

„Can I pool and crush several ticks together in one tube before testing?”

"Yes, but you have to make sure that each tick is crushed with its intestine and that you add enough buffer.“

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